Antibodies to Sperm-Associated Antigens Detected by Solid Phase Assays1
نویسندگان
چکیده
منابع مشابه
Human pancreatic cancer-associated antigens detected by murine monoclonal antibodies.
Two monoclonal antibodies, YPan1 and YPan2, were produced from spleen cells of mice immunized against a human pancreatic cancer cell line, Capan-2, which also reacted with eight other human pancreatic carcinoma cell lines and with sections of cancerous human pancreas tissue. Reactivity was also found with colonic and stomach carcinoma tissues. Whereas YPan1 reacted strongly with normal pancreat...
متن کاملCarbohydrate differentiation and cancer-associated antigens detected by monoclonal antibodies.
action was the pluripotential haemopoietic stem cell (CFUS ) . Whenever they were tested, free glycosaminoglycan chains were unable to reproduce P-D-xyloside influences on cell behaviour. On the basis of the cited biochemical evidence it seems likely that the xyloside effects are mediated through alterations in surface membrane proteoglycans. Most probably the xyloside-induced changes follow fr...
متن کاملColorectal carcinoma antigens detected by hybridoma antibodies.
Hybridoma cells which secrete colorectal carcinoma-specific antibodies have been produced and used to study the antigenic structure of these tumor cells. Nineteen antibodies have been studied in detail, and 15 of these are colorectal carcinoma specific. Only two antibodies reactive with carcinoembryonic antigen (CEA) have been discovered and five other antibodies that react with distinct epitop...
متن کاملMonoclonal antibodies to human sperm antigens.
To elucidate the molecular nature of human sperm autoantigens, attempts were made to raise monoclonal antibodies against these antigens, by hybridoma techniques. After successive immunizations with the particulate fractions of human sperm extract in BALB/c mice, the spleen cells were fused with P3-X63-Ag8 myeloma cells. Several clones and their subclones were obtained and shown by microplate ra...
متن کاملMonoclonal antibodies to human sperm antigens--II.
As part of our continuous effort to elucidate the biochemical and immunological nature of human sperm surface antigens, monoclonal antibodies to human spermatozoa were generated by improved hybridoma techniques. Following immunizations with the membrane fraction of human spermatozoa and cell fusions, hybrid cells were cultured in a semi-solid HAT-selection medium to maximize the number of monoc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1982
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod26.1.140